Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA

Attributes by original data submitter

Sample

source_name
HG fibroblasts_BRD4_ChIPSeq
cell type source
HGPS patient 1 (TRS-HGPS)
cell type
HGPS dermal fibroblasts transformed by retroviral introduction of TERT, HRAS and SV40 T antigens (TRS-HGPS1)
chip antibody
BRD4
chip antibody vendor
Bethyl labs

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Library construction was performed following Illumina TruSeq 2.0 protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
38340152
Reads aligned (%)
97.0
Duplicates removed (%)
20.8
Number of peaks
1044 (qval < 1E-05)

hg19

Number of total reads
38340152
Reads aligned (%)
95.9
Duplicates removed (%)
21.1
Number of peaks
412 (qval < 1E-05)

Base call quality data from DBCLS SRA